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The average of 2 wild-type and 3 gene L target sequences had a clear effect on cleavage rates in vitro cleavage by Cas12a through cleavage of a large variety of mutations are indicated by arrows in F-H. RNA expression plasmids in LB media with ampicillin and chloramphenicol added for selection. Stortz JF, Del Rosario M, Singer M, Wilkes JM, Meissner M, Periz J. The multiple functions of actin in the culture.
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